iii nb600 450  (Novus Biologicals)

Journal: International Journal of Nanomedicine

Article Title:

doi: 10.2147/ijn.s123839

Figure Lengend Snippet: Figure 5 Long-term intravenous exposure to c-SWNTs promotes deposition of collagens and ECM remodeling by upregulating both MMP-2 and TIMP-2. Notes: Immunohistochemical examination of Col I and Col III, MMP-2, and TIMP-2 (A) in the rat lungs after intravenous injection at indicated periods (positive signal: brown; c-SWNT aggregations: red arrows; magnification: 100×). Mean optical density of Col I, Col III, MMP-2, and TIMP-2 (B) based on immunohistochemical assay. Data represent mean ± SEM. *P,0.05, **P,0.01, and ***P,0.001 vs control. Abbreviations: c-SWNTs, carboxylated single-walled carbon nanotubes; ECM, extracellular matrix; MMP-2, matrix metalloproteinase-2; TIMP-2, tissue inhibitor of metalloproteinase-2; Col I, type-I collagen; Col III, type-III collagen; SEM, standard error of the mean.

Article Snippet: The proteins were blocked for 10 min in 5% bovine serum albumin in Tris buffered saline–Tween 20 (TBST; 25 mM Tris–HCl, pH 7.4, 125 mM sodium chloride, 0.05% Tween 20) and incubated with antibodies against Col III (1:1,000 dilution; Novus Biologicals, Littleton, CO, USA), NF-κB/p65 and inhibitor of kappa B alpha (IκBα) (1:1,000 dilution; Cell Signaling Technology, Danvers, MA, USA), tubulin (1:5,000 dilution; EarthOX Life Sciences, Millbrae, CA, USA), and glyceraldehyde 3-phosphate dehydrogenase (1:5,000 dilution; Multisciences, Hangzhou, People’s Republic of China) overnight at 4°C.

Techniques: Immunohistochemical staining, Injection, Control

Journal: International Journal of Nanomedicine

Article Title:

doi: 10.2147/ijn.s123839

Figure Lengend Snippet: Figure 6 SWNTs stimulate the synthesis of collagen and activate NF-κB signaling in rat lungs. Notes: Western blotting analysis of total level of Col III (A) and cytoplasmic levels of NF-κB/p65 and IκBα (B) after intravenous injection for 1, 7, and 30 days. Abbreviations: SWNTs, single-walled carbon nanotubes; NF-κB, nuclear factor-kappa B; Col III, type-III collagen; IκBα, inhibitor of kappa B alpha; c-SWNTs, carboxylated SWNTs.

Article Snippet: The proteins were blocked for 10 min in 5% bovine serum albumin in Tris buffered saline–Tween 20 (TBST; 25 mM Tris–HCl, pH 7.4, 125 mM sodium chloride, 0.05% Tween 20) and incubated with antibodies against Col III (1:1,000 dilution; Novus Biologicals, Littleton, CO, USA), NF-κB/p65 and inhibitor of kappa B alpha (IκBα) (1:1,000 dilution; Cell Signaling Technology, Danvers, MA, USA), tubulin (1:5,000 dilution; EarthOX Life Sciences, Millbrae, CA, USA), and glyceraldehyde 3-phosphate dehydrogenase (1:5,000 dilution; Multisciences, Hangzhou, People’s Republic of China) overnight at 4°C.

Techniques: Western Blot, Injection